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My
GSTP research aims to use novel mass spectrometric
instrumentation to characterize histone isoforms
in differentiating human embryonic stem cells.
Furthermore, we hope to correlate histone modification
isoforms with changes in gene expression in
hopes of better understanding how histone modifications
regulate transcription and differentiation.
Using a modified LTQ-Orbitrap mass spectrometer,
we have analyzed and quantified histone H4 isoforms
in human embryonic stem cells at multiple states
of differentiation, and have identified over
50 previously unknown protein isoforms. I will
be interrogating gene expression associated
with regions on DNA where specific isoforms
of histone H4 bind. I will be using ChIP-chip
analysis to idenfity genes associated with dimethylated
lysine 20. In addition, I will identify and
quantify changes in histone H3 with respect
to differentiation.
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